Higher yields of P1-phage recombinant plasmid DNA
نویسندگان
چکیده
منابع مشابه
Direct primer walking on P1 plasmid DNA.
The development of vectors such as P1, P1-derived artificial chromosome (PAC) or bacterial artificial chromosome (BAC) (2,6,8) has permitted the cloning of large DNA fragments. These vectors have become common in largescale sequencing and mapping projects. Therefore, a reliable primer walking protocol for P1 plasmids is desirable. It has been demonstrated previously that sequencing of P1 plasmi...
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The Cre DNA recombinase of bacteriophage P1 has become a useful tool for genomic manipulation in mice and other eukaryotes. Because Cre is of prokaryotic origin, the 38 kDa protein has been presumed to gain access to the eukaryotic nucleus simply because it is sufficiently small to pass through the nuclear pore by passive diffusion. Instead, we show here that Cre carries nuclear targeting deter...
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We have developed an in vitro DNA-replication system that replicates exogenously added mini-P1 plasmid DNA. The system consists of purified P1 RepA protein and a partially purified mixture of Escherichia coli replication proteins. It is essentially the same as that described for the replication of oriC plasmid DNA [Fuller, R.S., Kaguni, J.M. & Kornberg, A. (1981) Proc. Natl. Acad. Sci. USA 78, ...
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متن کاملRapid preparation and identification of insert-containing recombinant plasmid DNA.
The identification of transformant bacterial colonies containing potential recombinant DNA clones can be performed using a wide variety of screening methods including colony hybridization and autoradiography, polymerase chain reaction (PCR) and restriction endonuclease analysis of individual plasmid DNA (1,5). While colony hybridization can efficiently screen large numbers of recombinant clones...
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ژورنال
عنوان ژورنال: Technical Tips Online
سال: 1997
ISSN: 1366-2120
DOI: 10.1016/s1366-2120(08)70017-5